Ukusebenza okuphezulu kwe-Liquid Chromatography/HPLC
I-High Performance Liquid Chromatography/HPLC ikwabizwa ngokuba yi "high-pressure liquid chromatography", "high-speed liquid chromatography", "high-resolution liquid chromatography", "modern column chromatography", njl. njl. yechromatography.Isebenzisa ulwelo njengenqanaba elihambayo kwaye isebenzisa inkqubo yokufakwa koxinzelelo oluphezulu ukumpompa izinyibilikisi ezinee-polarities ezahlukeneyo okanye izinyibilikisi ezixubeneyo, ii-buffers kunye nezinye izigaba eziphathwayo ezinemilinganiselo eyahlukeneyo kwisigaba esimileyo.Emva kokuba amacandelo kwikholamu yechromatographic ahlulwe, afaka umtshina ukuze abonwe kunye nohlalutyo lwesampulu.Le ndlela ibe yinto ebalulekileyo yokwahlula kunye nokuhlalutya iteknoloji kwiinkalo zekhemistri, iyeza, imboni, i-agronomy, ukuhlolwa kwempahla kunye nokuhlolwa kwezomthetho.
Iimpawu zechromatography yolwelo olusebenzayo oluphezulu:
① Uxinzelelo oluphezulu: Isigaba esihambayo silulwelo.Xa ihamba kwikholamu yechromatographic, idibana nokuchasana okukhulu.Ukuze ukhawuleze udlule kwikholamu yechromatographic, i-carrier carrier liquide kufuneka igxininiswe.
② Ukusebenza okuphezulu: ukusebenza kakuhle kokwahlula.Isigaba esimileyo kunye nesigaba esihambayo sinokukhethwa ukufezekisa umphumo ongcono wokwahlula, ophindwe kaninzi ngaphezu kokusebenza kakuhle kokwahlulwa kwee-industrial distillation towers kunye ne-gas chromatography.
③Uvakalelo oluphezulu: Isixhobo se-UV sinokufikelela kwi-0.01ng.
④Uluhlu olubanzi lwezicelo: Ngaphezu kwe-70% ye-organic compounds inokuhlalutywa ngokusebenza okuphezulu kwe-chromatography yolwelo.
⑤ Isantya sohlalutyo olukhawulezayo kunye nesantya sokuhamba kolwelo olukhawulezayo: ngokukhawuleza kakhulu kunechromatography yolwelo lwakudala
Ukongeza, i-high-performance liquid chromatography kunye ne-chromatography columns zingasetyenziswa ngokuphindaphindiweyo, iisampuli azonakaliswa, kwaye kulula ukuzibuyisela.Noko ke, zikwanazo nezingeloncedo.Xa kuthelekiswa ne-chromatography yegesi, ngamnye unenzuzo yakhe kwaye ayancedisana.
Ixesha lokuposa: Nov-23-2023
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